| 1. | Influences of nitrogen ratio and carbon sources on adventitious shoots regeneration of malus zumi leaves in vitro
氮源比例和不同碳源对珠美海棠离体叶片再生不定芽的影响 |
| 2. | Coppice ( copse ) a woodland managed for wood production by cutting trees back to ground level at regular intervals ( usually 10 - 15 years ) and allowing adventitious shoots to grow up from the base
萌生林（杂树林） ：在森林更新作业中，由树木的定期伐桩（通常是10 15年）和根萌蘖而形成的森林。 |
| 3. | Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and 150mg / l kanamycin and incubated at 25 , 16 / 8h light / dark cycle . leaves of adventitious shoots were detected by gus staining
Sin旮16ea ）上暗培养3天后，移入加有抗生素150mg ikan和500mg几cr的诱芽培养基上诱导不定芽的分化。 |
| 4. | Adventitious shoots could be rooted and formed regenerated plants on 1 / 2 ms medium supplemented with naa0 . 4mg / l . hairy roots could be induced from the basal surface of the explants 8 days after inoculation of a . ficoidea cv . " ruliginosa " leaf explants with a . rhizogenes atcc 15834
红龙草叶片外植体被发根农杆菌atcc15834感染8d后，从形态学下端叶脉处陆续分化出乳白色的不定根， 21d后，叶片外植体的生根率高达92 . 5 。 |
| 5. | Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and loomg / l kanamycin and incubated at 25 , 16 / 8h light / dark cycle . small leaves of adventitious shoots differentiated from explants were cut and dipped into gus staining solution . positive shoots , gus tinted , were induced to root
分化出的不定芽切下半张叶片进行gus染色，呈阳性的植株从外植体上切下，在生根培养基（ ms 0刀sing lnaa 50mg几kan ）中诱导根的形成。 |
| 6. | The regeneration system of tobacco was established . the adventitious shoots were induced from leaf explants of tobacco based on ms basal medium supple - - mented with 2 . 0mg / l 6 - ba and 0 . 3mg / l naa . then the regenerated plants were rooted on ms medium containing 0 . 3mg / l naa
植株再生体系的建立采用无菌苗烟草叶片为外植体，以ms为基本培养基，筛选出ms + 6 - ba2 . 0mg l + naa0 . 3mg l以诱导不定芽的分化，并于ms + naa0 . 3mg l的pa硕士学位论文v沉了砂“工队nk ”主根培养基上生根，获得完整再生植株。 |
